工业微生物2011,Vol.41Issue(2):47-50,4.DOI:10.3969/j.issn.1001-6678.2011.02.010
融粘帚霉对葛根素的生物转化研究
Biotransformation of puerarin into 3′-hydroxypuerarin by Gliocladium deliguescens
摘要
Abstract
The major product of microbial transfonnation of puerarin by Cliocladium deligue.scen.s AS3 .3987 was identified as 3' :hydroxypuerarin on the basis of its spectral data. 3' -hydroxypuerarin was directly determined by HPLC . Methanol and water (27: 73) was used as the mobile phase on C18 column and the UV detection wavelength was 254 nm . The maximum transformation rate obtained was 93 .2 mg 3' -hydroxypuerarin/g puerarin at 30℃, 160 r/min for 36 h with the substrate concentration of 400 μg/mL .关键词
高效液相色谱法/微生物转化/葛根素/3′-羟基葛根素/融粘帚霉引用本文复制引用
杨莉萍,崔志超,高芳,张新,叶辉..融粘帚霉对葛根素的生物转化研究[J].工业微生物,2011,41(2):47-50,4.基金项目
江苏省南通市科技计划财政资金资助项目(K2009050). (K2009050)