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融粘帚霉对葛根素的生物转化研究

杨莉萍 崔志超 高芳 张新 叶辉

工业微生物2011,Vol.41Issue(2):47-50,4.
工业微生物2011,Vol.41Issue(2):47-50,4.DOI:10.3969/j.issn.1001-6678.2011.02.010

融粘帚霉对葛根素的生物转化研究

Biotransformation of puerarin into 3′-hydroxypuerarin by Gliocladium deliguescens

杨莉萍 1崔志超 1高芳 1张新 1叶辉1

作者信息

  • 1. 南通大学生命科学学院,南通,226007
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摘要

Abstract

The major product of microbial transfonnation of puerarin by Cliocladium deligue.scen.s AS3 .3987 was identified as 3' :hydroxypuerarin on the basis of its spectral data. 3' -hydroxypuerarin was directly determined by HPLC . Methanol and water (27: 73) was used as the mobile phase on C18 column and the UV detection wavelength was 254 nm . The maximum transformation rate obtained was 93 .2 mg 3' -hydroxypuerarin/g puerarin at 30℃, 160 r/min for 36 h with the substrate concentration of 400 μg/mL .

关键词

高效液相色谱法/微生物转化/葛根素/3′-羟基葛根素/融粘帚霉

引用本文复制引用

杨莉萍,崔志超,高芳,张新,叶辉..融粘帚霉对葛根素的生物转化研究[J].工业微生物,2011,41(2):47-50,4.

基金项目

江苏省南通市科技计划财政资金资助项目(K2009050). (K2009050)

工业微生物

OACSCDCSTPCD

1001-6678

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