南方农业学报2011，Vol.42Issue(1)：105-108,4.
一种检测乳过氧化物酶方法的建立
Development of a method to detect lactoperoxidase( LP ) activity in milk
摘要
Abstract
[Objective]The present experiment was conducted to develop a simple, rapid and accurate method for detection of lactoperoxidase(LP) activity in milk samples. [Method]Using 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) as the substrate, the method to detect LP activity in milk was established by determining the reaction kinetics. The reaction conditions were optimized by observing effects of different incubation periods, pH values, substrate and H202 concentrations and incubation temperatures. [Result]The optimized conditions for reaction were as follows: 2 mmoi/L of substrate concentration, 5 mmol/L of H202. pH 5.5, and 100 sec of response time, followed by the measurement at 416 nm wavelength. The coefficient of variation in the same group was found to be 2.39％ and that of the different group was 3.42％. The correlation coefficient was 0.9953, and the accuracy rating did not exceeded beyond 5％. [Conclusion]Besides being the simple and rapid, the developed ABTS method was found to be highly accurate and precise on detection of LP compared to the other methods.关键词
乳过氧化物酶/ABTS法/分光光度计/吸光度值Key words
lactoperoxidase/ ABTS/ kinetics/ absorbance分类
生物科学引用本文复制引用
惠永华,王路,牛金涛..一种检测乳过氧化物酶方法的建立[J].南方农业学报,2011,42(1):105-108,4.基金项目
河南省重大科技攻关项目(0522010800) （0522010800）
