南方农业学报2011，Vol.42Issue(1)：11-15,5.
桑树二倍体及其人工诱导同源四倍体遗传差异的RAPD分析
Assessment of RAPD polymorphism amongst mulberry diploids and their homologous tetraploids
摘要
Abstract
[Objective]The experiment was conducted to analyze the genetic variation amongst different mulberry diploids and their homologous tetraploids to understand the genetic mechanism of multagenesis in mulberry polyploids.[Method]The polymorphism amongst diploid and homologous tetrapioid of 8 mulberry varieties was assessed using RAPD molecular marker technique and the genetic diversity was analyzed using cluster analysis. [Results]The results showed that RAPD polymorphism between diploid and their homological tetraploid varied significantly in different mulberry varieties.Differential bands were not observed amongst diploid and their homological tetraploid in Nongsang 8 and Husang 199 varieties, while the highest RAPD polymorphism (20.41％) between diploid and its homological tetraploid was observed in Husang 32, followed by Tongxiangqing, Huangsang 37, Husang 197, Guosang 20 and Yu 71-1 ,which was 9.09, 6.06, 5.45,5.17 and 3.17％,respectively. The cluster analysis results clearly indicated the presence of two distinct clusters of the mulberry varieties used in the assessment. Husang 32 and Tongxiangqing were found in the same cluster. [Conclusion]The colchicine treatment can cause a significant genetic variations in different diploids of mulberry genotypes.关键词
桑树/二倍体/同源四倍体/遗传差异/RAPDKey words
mulberry/ diploid/ homologous tetraploid/ genetic variation/ RAPD分类
农业科技引用本文复制引用
林强,邱长玉,朱方容,赵卫国,陈小青,方荣俊,朱光书,李乙,曾燕蓉..桑树二倍体及其人工诱导同源四倍体遗传差异的RAPD分析[J].南方农业学报,2011,42(1):11-15,5.基金项目
现代农业产业技术体系建设专项项目(nycytx-27-gw105) （nycytx-27-gw105）
广西科学研究与技术开发计划项目(桂科攻0992012) （桂科攻0992012）
广西自然科学基金项目(桂科自0991177) （桂科自0991177）
