南方农业学报2011，Vol.42Issue(1)：86-89,4.
凡纳滨对虾肌肉组织cDNA全长文库的构建
Construction of the full length cDNA library from muscular tissue of Litopenaeus vannamei
摘要
Abstract
[Objective]The studies had been undertaken in order to understand the biological basis of expression of functional genes in muscular tissue of Litopenaeus vannamei. [Method]The full length cDNA library from muscular tissue of Litopenaeus vannmei was constructed and expressed Sequence Tags (ESTs) were sequenced. [Result]The constructed library was 8.50×106 CFU in capacity with 95％ recombinant coefficient. The PCR results showed that the inserts ranged from 0.5 to 4.0 kb and most of them were larger than 1.0 kb. 72 clones were randomly selected and sequenced for full length. Of which, 37 cDNA sequences were identified with known functions, and 18 cDNA sequences remained as unidentiffed. Using gene ontology function classification, 37 cDNA sequences with known function were classified into groups of protein synthesis, cytoskeleton, signal transduction, metabolism, transporter, energy, transcription factors, response to disease/defense, reproduction development and unknown function,respectively. The protein synthesis-related genes were the highest in number (27.03％), followed by cytoskeleton (13.51％), signal transduction (13.51％), and metabolism (13.51％), which collectively accounted for 67.56％ of the total identified genes. [Conclusion]The information in constructed full length cDNA library of Litopenaeus vannmei muscular tissue can be used in further studies related to the expression and function of genes in Litopenaeus vannmei.关键词
凡纳滨对虾/肌肉组织/cDNA全长文库/生长性状Key words
Litopenaeus vannmei/ muscular tissue/ cDNA library/ growth traits分类
农业科技引用本文复制引用
熊建华,高永华,马宁,盛小伟,陈晓汉..凡纳滨对虾肌肉组织cDNA全长文库的构建[J].南方农业学报,2011,42(1):86-89,4.基金项目
国家科技支撑计划项目(2008BADB9B03) （2008BADB9B03）
国家虾产业技术体系岗位科学家经费项目(nycytx-46) （nycytx-46）
广西直属公益性科研院所基本科研项目(2060302GXIF-2008-02) （2060302GXIF-2008-02）
广西自然科学基金项目(桂科自0991106) （桂科自0991106）
