南方农业学报2011，Vol.42Issue(1)：1-5,5.
采用分生组织培养生产脱甘蔗黄叶病毒苗
Regeneration of Sugarcane yellow leaf virus free sugarcane plantlets through meristem culture
Mishra S 1Rao G P1
作者信息
- 1. Kunraghat甘蔗试验站,印度戈勒克布尔 273008
- 折叠
摘要
Abstract
[Objective]The present study is an attempt to produce Sugarcane yellow leaf virus (SCYLV) free planters from commercial sugarcane varieties, UP 01235 and UP 0097, using meristem tip culture technique. [Method]Plantlets were produced using meristem (different size) as explant, collected from virus infected and healthy field grown sugarcane plants. RT-PCR assay of regenerated plantlets was carried out to check the presence of virus at monthly interval up to six month stage using specific primer pair (SCYLV-615F: 5'-ATGAATACGGGCGCTAACCGYYCAC-3' and SCYLV-615 R: 5'-GT-GTIGGGGRAGCGTCGCYTACC-3') obtained from Sugarcane yellow leaf virus. [ Result ]The plantlets of both varieties,regenerated from the meristems measuring up to 1.5 mm, did not showed the presence of virus during any growth stage under glasshouse conditions, this indicated the absolute elimination of SCYLV. However, the meristems measuring more than 1.5 mm size showed presence of the SCYLV in PCR assay.[Conclusion]More than 200 plantlets, obtained from 1.0～ 1.5 mm meristem tip culture were propagated in greenhouse showed absence of SCYLV during all growth stages.关键词
RT-PCR/分生组织培养/甘蔗/甘蔗黄叶病毒/脱毒苗Key words
RT-PCR, meristem culture/ sugarcane/ Sugarcane yellow leaf virus/ virus free plantlets分类
农业科技引用本文复制引用
Mishra S,Rao G P..采用分生组织培养生产脱甘蔗黄叶病毒苗[J].南方农业学报,2011,42(1):1-5,5.
