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毒莠定对郁金香愈伤组织诱导及胚状体产生的影响

周音 张智奇 张建军 顾佳清

上海农业学报2011,Vol.27Issue(1):7-10,4.
上海农业学报2011,Vol.27Issue(1):7-10,4.

毒莠定对郁金香愈伤组织诱导及胚状体产生的影响

Effects of picloram on tulip callus induction and somatic embryogenesis

周音 1张智奇 1张建军 1顾佳清1

作者信息

  • 1. 上海市农业科学院林木果树研究所,上海,201106
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摘要

Abstract

The effects of 3 reagents such as picloram on tulip callus induction and somatic embryogenesis were studied by using the optimal design of "311-B" regression.The results showed that the callus inductivity and the somatic embryogenesis had a very significant correlation with picloram concentration.Picloram played a decisive role in tulip callus induction,and the callus could occur through 20 d culture.The optimal medium was MS+6 mg/L picloram+0.5 mg/L BA,the callus inductivity reached 100% and each piece of the callus averagely produced 10 embryoids.Picloram and BA had a positive interaction and could be used together.But picloram had a negative interaction with hymexazol and both did not have to be used together.

关键词

郁金香/毒莠定/组织培养/愈伤组织/胚状体/回归最优设计

分类

农业科技

引用本文复制引用

周音,张智奇,张建军,顾佳清..毒莠定对郁金香愈伤组织诱导及胚状体产生的影响[J].上海农业学报,2011,27(1):7-10,4.

基金项目

上海市农委重点科技攻关项目[(2007)第1-1号]资助 (2007)

上海农业学报

OA北大核心CSCDCSTPCD

1000-3924

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