上海农业学报2011，Vol.27Issue(1)：55-59,5.
一种果梅休眠枝韧皮部DNA提取方法
A method of extracting DNA from the phloem of dormant wood of Prunus mume
摘要
Abstract
The phloems of 5 Prunus mume cultivars ' dormant woods were used as test materials,their genomic DNAs were extracted by the improved CTAB method,and the effect of DNA extraction from the phloems was contrasted with that from their young leaves.The results showed that all the genomic DNAs extracted from the phloems by this method were rather good in both purity and integrality , the D260 nm/D280 nm ratio was 1.8～2.0 , no degradation occurred, the RNA was eliminated completely ,and the DNAs could be completely digested by restriction endonucleases.The SSAP analysis indicated that the electrophoretic phenotype of the phloem DNA was just the same as that of the leaf DNA(the primer combination: LTR-1/EcoR-ACC, LTR-2/EcoR-ACC), being clear in band and good in polymorphism.It was concluded that the total DNA extracted by this method from the phloem of dormant wood of P.mume was completely suitable for the SSAP analysis.关键词
果梅/DNA提取/韧皮部/SSAP分类
生物科学引用本文复制引用
王飞,沈玉英,佟兆国,高志红,章镇..一种果梅休眠枝韧皮部DNA提取方法[J].上海农业学报,2011,27(1):55-59,5.基金项目
国家自然科学基金(30671438 （）
30871681)资助 （）
