厦门大学学报（自然科学版）2011，Vol.50Issue(1)：97-100,4.
E-cadherin前体蛋白抗体的制备与鉴定
Preparation and Identification of the Antibody Against the Precursor Protein of E-cadherin
摘要
Abstract
E-cadherin,an important tunor suppressor which was defected highly correlates with tumor metastasis was derived from its precursor protein. As for the commercial unavailability,the precursor form of E-cadherin can not be detected at endogenous levels.In order to get qualified antibody agianst E-cadherin pre,Pst Ⅰ-Pvu Ⅱ fragment (amino acids 31 to 164) N-terminal in the precursor region of E-cadherin was ligated to pGST-parallel vector,expressed in the E. coli. strain BL-21. The fusion protein was purified by GST beads, and rabbits were immunized. 9 weeks later, the sera of the rabbits were harvested and an anti-Pre-E-cadherin polyclonal antibody was purified. The precursor of E-cadherin was successfully detected with great sensitivity and specificity by western blotting using this anti-Pre-E-cadherin antibody in the lysate of 293T cells transfected with pCMV5-c-myc E-cadherin. Furthermore, the antibody lays a good foundation for further research on the function of E-cadherin pre,especially on its role in signal transduction pathways.关键词
E-cadherin pre/抗体/pGST E-cadherin pre分类
医药卫生引用本文复制引用
王继峰,叶志云,林舒勇..E-cadherin前体蛋白抗体的制备与鉴定[J].厦门大学学报（自然科学版）,2011,50(1):97-100,4.基金项目
福建省自然科学基金资助项目(2008J0109) （2008J0109）
