厦门大学学报（自然科学版）2011，Vol.50Issue(1)：123-126,4.
4种促性腺激素释放激素抗体的制备、鉴定和纯化
Preparation,Identification and Purification of Antibodies Against Four GnRHs
摘要
Abstract
Four immunogens was prepared by conjugation of four synthetic GnRH peptides ( octopus GnRH , tunicate GnRH- Ⅰ , lamprey GnRH- Ⅰ and lamprey GnRH-Ⅲ) and carrier protein key-hole limpet hemocyanin (KLH) respectively by MBS method. Antibody production against each immunogen was initiated in vivo by emulsifying them in Freund's adjuvant and administering them to 2-month-old male New Zealand rabbits by hypodermal injection The tirer of antiserums was tested by indirect ELISA,and the results showed that the titer of four antibodies was 1 : 500 000. The results of Western blot analysis showed that each antibody could specifically bind to the corresponding GnRH peptide. Finally, antiserums were purified by affinity purification, and the purified antibody showed a single band in SDS-PAGE. These datas suggest that four GnRH antibody with high effect and high purity were produced,and they provide an useful tool for studying GnRH of lower animal.关键词
GnRH/多肽/多克隆抗体/亲和纯化分类
医药卫生引用本文复制引用
冯碧云,黄辉洋,叶海辉,尚丽丽,李少菁..4种促性腺激素释放激素抗体的制备、鉴定和纯化[J].厦门大学学报（自然科学版）,2011,50(1):123-126,4.基金项目
国家自然科学基金资助项目(41076081) （41076081）
