核农学报2011，Vol.25Issue(1)：43-47,5.
空竹悬浮细胞系的建立
ESTABLISHMENT OF SUSPENSION CELL LINE OF Cephalostachyum fuchsianum
摘要
Abstract
Cell suspension culture of bamboo is difficult. The suspension cell line of Cephalostachyum fuchsianum was established for the first time in this study. Seed embryos of Cephalostachyum fuchsianum were used to induce calli. The calli were dedifferentiated from the embyos on the medium of 3/4MS + 500mg/L proline + 500mg/L glutamine + 300mg/L tryptone +3.0mg/L 2,4- D + 1.5mg/L KT + 30g/L sucrose + 5g/L agar. A fine suspension cell line could be established by inoculating the calli in the medium of MS + 500mg/L proline + 500mg/L glutamine + 300mg/L tryptone +1.5mg/L 2,4 - D + 30g/L sucrose after 3 times shaking subcultures. The subculture cycle was 9d. The calli could be acquired from suspension cell line after the cells being cultured for 25d in the solid medium, and the appropriate inoculation density was 20g/L.关键词
空竹/悬浮细胞/细胞培养Key words
Cephalostachyum fuchsianum/ suspension cells/ cell culture引用本文复制引用
姚娜,岳晋军,顾小平,杨凯,李潞滨..空竹悬浮细胞系的建立[J].核农学报,2011,25(1):43-47,5.基金项目
浙江省重大科技专项重点项目(No.2009C12097),十一五科技支撑项目(2006BAD19B08),中国林业科学研究院亚热带林业研究所基金项目(No.RISF6908),林业公益性行业科研专项(No.200704001) （No.2009C12097）
