核农学报2011，Vol.25Issue(1)：48-52,5.
γ辐照对'巴西蕉'体细胞胚发生的影响
EFFECT OF 60Co γ-RAYS IRRADIATION ON SOMATIC EMBRYOGENESIS OF Musa AAA CAVENDISH cv. ‘Baxijiao’
摘要
Abstract
Immature male flowers from 60Co γ-rays irradiated ‘ Baxijiao’ (Musa AAA Cavendish) were selected as explants for callus induction, followed by establishment of embryogenic cell suspension (ECS) and plant regeneration via somatic embryogenesis. The effect of irradiation on the plant regeneration capacity of ECS was also studied. The results showed that the irradiation doses of 20 to 80Gy could significantly increase embryogenic callus induction frequency. After 3 months selection and subculture, good ECS with high regeneration capacity were obtained. Similarly,20 to 80Gy irradiation could also significantly increase the plant regeneration capacity of the ECS, which suggested that low dose irradiation was good for somatic embryogenesis of ‘ Baxijiao’.关键词
香蕉/60Co γ射线辐照/愈伤组织诱导/胚性细胞悬浮系/体胚发生/植株再生能力Key words
banana/ 60Co γ-rays irradiation/ callus induction/ embryogenic cell suspension/ somatic embryogenesis/plant regeneration capacity引用本文复制引用
徐春香,谢丽君,陈厚彬,胡桂兵,李华平,林贵美..γ辐照对'巴西蕉'体细胞胚发生的影响[J].核农学报,2011,25(1):48-52,5.基金项目
现代农业产业技术体系建设专项(nycytx--33),广东省自然科学基金项目(07006698),农业部行业专项(nyhyzx07-029),广东省科技攻关(2010B020305004) （nycytx--33）
