核农学报2011，Vol.25Issue(1)：6-13,8.
小麦RPL21基因同源克隆与表达分析
CLONING AND EXPRESSION ANALYSIS OF RPL21 GENE IN WHEAT (Triticum aestivum L. )
摘要
Abstract
To understand the genome structure and expression patterns of 60S ribosomal protein L21 (RPL21), partial sequence of RPL21 gene from the GenBank database was used as a query probe to blast the GenBank database. Based on the assembled homologous cDNA sequence, both cDNA and DNA sequences of RPL21 from multi-ovary lines were isolated and characterized by RT-PCR. Furthermore, differential expression of genes between the multi-ovary line and mono-ovary line, expression patterns of genes in different development stages of ears and different tissues of multi-ovary line were analyzed by semi-quantitative RT-PCR. The cDNA sequence (GenBank accession No. HM138480) was 521bp in length and the open reading frame encoded a peptide of 164 amino acids. The DNA sequence ( GenBank accession No. HM063421) was 1600bp in length, which contained two exons and one intron. According to the expression analysis, the expression of the gene in 2 ～ 4mm ears of multi-ovary line was higher than that in mono-ovary line. This gene was upregulated with the development of young ear of multi-ovary line, and arrived at the highest expression levels at 8 ～9mm young ear stage. It was speculated that more RPL21 were needed for the growth and development of floral organ. Besides, the expression patterns of the gene in different tissues of multi-ovary lines were in the order of stem shoot meristem ≈ young root ＞ young ear ＞ young leaf, indicating that they were abundantly expressed in various tissues, expression levels of RPL21 genes was the highest in rapidly growing tissues.关键词
多子房小麦/RPL21基因/克隆/表达分析Key words
multi-ovary wheat/ RPL21 gene/ clone/ expression analysis引用本文复制引用
栗现芳,马守才,张改生,牛娜..小麦RPL21基因同源克隆与表达分析[J].核农学报,2011,25(1):6-13,8.基金项目
国家863计划重大专项(2009AA101102),国家自然科学基金(31071477),高等学校博士点基金(20090204110024),陕西省13115科技创新工程重大科技专项(2007ZDKG-020),国家杨凌农业生物技术育种中心专项基金(99-1A),西北农林科技大学拔尖人才支持计划项目 （2009AA101102）
