核农学报2011，Vol.25Issue(1)：20-25,42,7.
棉花GhBI-1基因全长cDNA的克隆与表达分析
FULL-LENGTH cDNA CLONING AND ANALYSIS OF GHBI-1 FROM UPLAND COTTON (Gossypium hirsutum L.)
摘要
Abstract
In higher plants and animals, BI-1 (Bax inhibitor-1) is the inhibitor of apoptosis.Overexpression of BI-1 in plant can improve the tolerance to cell death induced by biological and abiotic stress.Using known BI-1 gene sequences of upland cotton, two full-length cDNAs of GhBI gene, GhBI-la and GhBI-lb, were amplified by RACE technology.The homology of these two genes in nucleotides was 86％ and was 87％ in encoded protein.GhBI-la has six transmembrane regions while GhBI-lb has seven transmembrane regions.GhBl-la and GhBl-lb were expressed both in roots and leaves.GhBl-lb was induced by Verticillium dahliae, but GhBI-la had no significant difference after inoculation.Homologous analysis suggested that GhBI-1a located in subgenome D and GhBI-1b located in subgenome A of the allotetroploid cotton genome.Cloning of these two genes would be benefited to further study the biotic and abiotic stress adaptation mechanisms of upland cotton and thus to improve the tolerance in cotton breeding.关键词
陆地棉/GhBI-1基因/全长cDNA/RACEKey words
Gossypium hirsutum/ GhBI-1 gene/ full-length cDNA/ RACE引用本文复制引用
郑玲,张玉芹,张传云,刘国栋,王芙蓉,张军..棉花GhBI-1基因全长cDNA的克隆与表达分析[J].核农学报,2011,25(1):20-25,42,7.基金项目
国家转基因生物新品种培育重大专项(2009ZX08005-018B),国家863计划(2006AA10A108),山东省农业良种工程重大课题(2007LZ005-06) （2009ZX08005-018B）
