厦门大学学报（自然科学版）2011，Vol.50Issue(1)：76-81,6.
蛋白质、胆固醇对卵磷脂囊泡稳定性的影响
Effect of Protein and Cholesterol on the Phosphatidylcholine Vesicle
摘要
Abstract
Biological membrane with phosphatidylcholine,cholesterol and protein (bovine serum albumin,BSA) was simulated. The mixed monolaver at the air/water interface of phosphatidylcholine,cholesterol and BSA was investigated by Langmuir balance technique. The Zeta potential of cholesterol, phosphatidylcholine, BSA and their mixtures were investigated. The excess area of mixed monolyer and Zeta potential showed that there were interactions of mutual attraction among phosphatidylcholine, cholesterol and BSA. The interactions made the mixed vesicle more stable. The effect of cholesterol and BSA on the structure transformation of phosphatidylcholine vesicle was studied by means of kinetic and TEM. Gemini surfactants intercalated the bilayer via electrostatic interaction and hydrophobic effect and made the vesicle breakdown as a result. The E(a) was calculated from the change of turbidity at 25 , 30 ,35 ,40 and 45 ℃. Comparing with previous results,the E(a) of phosphatidylcholine, cholesterol and BSA was larger than phosphatidylcholine vesicle,and between those of phosphatidylcholine vesicle with cholesterol and phosphatidylcholine vesicle with BSA. On the other hand,it indicated that the phosphatidylcholine vesicles with cholesterol and( or) BSA were more stable to Gemini surfactants than phosphatidylcholine vesicle and harder to be brokendown.关键词
卵磷脂囊泡/胆固醇/牛血清白蛋白/Gemini表面活性剂分类
化学化工引用本文复制引用
高素君,徐晓明,黄铭湘,蓝琴,韩国彬..蛋白质、胆固醇对卵磷脂囊泡稳定性的影响[J].厦门大学学报（自然科学版）,2011,50(1):76-81,6.基金项目
厦门大学科技创新重点基金项目(XMKJCX20052001) （XMKJCX20052001）
