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不同恶化程度胃癌细胞系的细胞间隙连接通讯功能及Cx43表达的差异

田志华丁慧荣张宏张伟李振甫

Journal of Beijing Normal University (Natural Science Edition)2011，Vol.47Issue(1):63-68, Sheet 2, 7.

Journal of Beijing Normal University (Natural Science Edition)2011，Vol.47Issue(1):63-68, cover 2,7.

不同恶化程度胃癌细胞系的细胞间隙连接通讯功能及Cx43表达的差异

DIFFERENT FUNCTIONS OF GAP JUNCTION INTERCELLULAR COMMUNICATION AND EXPRESSION OF GAP JUNCTION PROTEIN CONNEXIN 43 IN VARIOUS HUMAN GASTRIC CANCER CELL LINES WITH DIFFERENT MALIGNANT DEGREE

田志华 ¹丁慧荣 ¹张宏 ¹张伟 ²李振甫¹

作者信息

1. 北京大学临床肿瘤学院,北京肿瘤医院暨北京市肿瘤防治研究所中心实验室,恶性肿瘤发病机制及转化研究教育部重点实验室,100142,北京
2. 北京师范大学生命科学学院,细胞增殖及调控生物学教育部重点实验室,100875,北京
折叠

Abstract

To correlate function of gap junction intercellular communication (GJIC) and connexin43 (Cx43) expression with carcinogenesis, progression and metastasis of gastric cells GES-1 (human normal gastric mucosal cell line), AGS (low malignant gastric cell line), SGC-7901 (middle malignant gastric cell line), BGC-823 (high malignant gastric cell line), GJIC function and Cx43 expression at mRNA and protein levels were investigated using fluorescence recovery after photobleaching (FRAP) under laser scanning confocal microscope, scrape-loading dye transfer (SLDT), real time quantitative PCR and indirect immunofluorescence assay. Data showed that with increasing malignancy, GJIC function become reduced or lost. GES-1 showed good GJIC function, AGS, SGC-7901 showed reduced GJIC function, but BGC-823 showed no GJIC function.Compared to GES-1, Cx43 expression (mRNA and protein) was downregulated in AGS and SGC-7901, but absent in BGC-823. This suggests that reduction in GJIC function and Cx43 expression was associated with occurrence, development and metastasis of gastric tumor.

关键词

胃癌细胞系/细胞间隙连接通讯(GJIC)/间隙连接蛋白(Cx43)/荧光漂白恢复技术/划痕标记荧光染料示踪技术/荧光定量PCR技术

Key words

gastric cancer cell lines/ GJIC/ Cx43/ fluorescence recovery after photobleaching/ scrape`loading dye transfer/ real time quantitative PCR

引用本文复制引用

田志华,丁慧荣,张宏,张伟,李振甫..不同恶化程度胃癌细胞系的细胞间隙连接通讯功能及Cx43表达的差异[J].北京师范大学学报（自然科学版）,2011,47(1):63-68,封2,7.

基金项目

北京肿瘤医院院内基金资助项目(10-29) （10-29）

北京师范大学学报（自然科学版）

OA北大核心CSCDCSTPCD

ISSN：0476-0301

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