动物学研究2011，Vol.32Issue(1)：56-61,6.DOI:10.3724/SP.J.1141.2011.01056
应用改良流式法检测猕猴和食蟹猴体内血型抗体水平的分布情况
Application of flow cytometry to detect ABO blood group antibody levels in rhesus monkeys and cynomolgus monkeys
摘要
Abstract
Similar to humans, most of non-human primates also contain the ABO blood group system. In this study,we sought to evaluate the ABO antibody (Ab) levels in monkeys using a modified flow cytometry method (FCM). The standard commercial human A or B-red blood cells (RBCs) were used as target cells. The binding of target cells and anti-A or B blood group Ab in sera of thesus or cynomolgus monkeys was detected by flow cytometry after adding secondary specific fluorescence-labeled anti-human IgG or IgM Ab. Human healthy blood donor sera were used as controls. The results revealed that, using clear monkey sera, which were pre-absorbed on normal human type O RBCs to remove non-specific anti-human Abs, the modified FCM gave an accurate detection of ABO Ab levels in monkeys.When compared with the results of human sera, the distribution of ABO Ab levels in monkey sera were significantly lower (P＜0.05). We concluded that the modified FCM could be used for the detection of monkey ABO Ab levels. The technique and data will be very valuable for the future establishment of ABO-incompatible organ transplant models in non-human primates, which could improve clinical applications.关键词
流式检测法/ABO血型抗体/猕猴/食蟹猴分类
医药卫生引用本文复制引用
张弛,王筱啸,王璐,向莹,魏晴,王婉瑜,熊郁良,陈实,陈刚..应用改良流式法检测猕猴和食蟹猴体内血型抗体水平的分布情况[J].动物学研究,2011,32(1):56-61,6.基金项目
国家自然科学基金面上项目(30872384) （30872384）
