动物学研究2011，Vol.32Issue(1)：50-55,6.DOI:10.3724/SP.J.1141.2011.01050
六个肥胖相关基因在食蟹猴2型糖尿病不同发病时期的差异表达
Differential expression of six obesity-related genes with different disease phases of T2DM in cynomolgus monkey
摘要
Abstract
Type 2 diabetes mellitus (T2DM) is a metabolic disease with a strong genetic component that is very prevalent in the world. The aim of this study is to investigate the association of a set of six obesity-related genes with the different disease phases of T2DM in a model using middle or aged cynomogus monkeys. A total of 25 male monkeys were used and fed with high-fat diet (15％ lard). The disease development and progression of T2DM were monitored through the levels of plasma glucose and lipid. The mRNA expression of 6 genes was evaluated using real-time PCR on monocyte isolated from monkey peripheral blood. The 2-hour plasma glucose levels followed oral glucose tolerance test (OGTT) were (11.06±6.05) mmol/L and (13.12±2.89) mmol/L respectively (P＜0.01), and the fasting plasma glucose level was (7.58±1.56) mmol/L (vs controls, P＜0.01), indicating that we developed successful the models of pre-diabetic and diabetic disease in the cynomolgus monkey. Of the six tested genes, CDKN2B, IGF2BP2, and FTO genes were significantly up-regulated with disease progression in T2DM. We found that the expression of IGF2BP2 and FTO increased 65.92 and 4.30 folds in the developed T2DM. We conclude that the genes of CDKN2B, IGF2BP2, and FTO can be used as early diagnostic and prognostic biomarkers in type 2 diabetes.关键词
食蟹猴/2型糖尿病/肥胖相关基因/基因表达Key words
Cynomolgus monkey/ Type 2 diabetes/ Obesity-related genes/ Gene expression分类
医药卫生引用本文复制引用
靳丽莎,郝香芬,彭白露,张艳春,万玉玲,季芳,夏机良,刘晓明..六个肥胖相关基因在食蟹猴2型糖尿病不同发病时期的差异表达[J].动物学研究,2011,32(1):50-55,6.基金项目
广州市科技计划项目大型实验动物国际认证及外包服务(2009A1-E051) （2009A1-E051）
广东省林业局项目实验猴繁育关键技术研究及产业示范(2009KJCX016) （2009KJCX016）
"重大新药创制"科技重大专项"十二五"计划 （）
人类重大疾病灵长类动物模型资源平台的建设(2011ZX09307-303-03) （2011ZX09307-303-03）
