草业学报2011，Vol.20Issue(1)：125-130,6.
甘肃红砂不同种群遗传多样性的ISSR分析
Analysis of genetic diversity in Reaumuria soongorica populations in Gansu using ISSR markers
摘要
Abstract
Genetic diversity and genetic structure of 5 Reaumuria soongorica populations from Gansu (total about 50 individuals), were analyzed by ISSR (inter simple sequence repeat) markers. Twelve highly stable and repeatable ISSR primers (selected from 50 primers tested) detected a total of 69 loci of which 60 were polymorphic (86.96％ of the total). The PCR products ranged from 500 to 3 000 bp. At the species level, the Shannon index (I) was 0. 542 9, Nei's gene diversity index (H) was 0. 379 0, the coefficient of gene differentiation (Gst) was 0. 096 4, and the gene flow (Nm) was 4. 685 1. The software Popgen 32 analysis of the results showed that there was more genetic differentiation within populations than between populations. At the population level, I and H were 0. 489 3 and 0. 342 4, respectively. All of these results showed that R. soongorica has a high genetic diversity in Gansu. Cluster analysis showed no direct correlation between genetic distance and geographic distance, suggesting that genetic diversity of R. soongorica in Gansu was possibly related to species traits and communities.关键词
甘肃红砂/遗传多样性/遗传距离Key words
Reaumuria soongorica/ genetic diversity/ genetic distance分类
农业科技引用本文复制引用
冯亮亮,唐红,李毅,马彦军,苏世平..甘肃红砂不同种群遗传多样性的ISSR分析[J].草业学报,2011,20(1):125-130,6.基金项目
国家林业局重点科研项目(2006-35)和甘肃省科技攻关项目(2GSO64-A41-003-01)资助. （2006-35）
