草业学报2011,Vol.20Issue(1):140-147,8.
玉米胚乳突变基因ae连锁累赘的SSR分析
SSR analysis of the linkage drag of endosperm mutation gene ae
摘要
Abstract
Molecular assisted selection was used to analyse linkage drag of different recurrent parents on two flanks of two populations and for restoring the ratio of two populations by SSR markers for ae. These are evenly distributed on chromosome 5. Linkage drags at the two flanks were controlled in 13.2 and 17.2 cM of CHBC1F2 populations by SSR markers and the linkage drag at one flank was controlled in 13.9 cM of DHBC1F2 populations. Genetic background resemblance of individuals of the CHBC1 F2 population to the recurrent parent's was 85.7%. Genetic background resemblance of individuals of the DHBC1 F2 population to the recurrent parent's was 87.5%. It is possible to identify recombination individuals with the targeted gene through a high density (10-20 cM per marker) map. This reduced unnecessary fragmentation of chromosomes and eliminated linkage drag of the targeted gene in backcross breeding. The hitchhiking effect may have been reduced.关键词
分子标记辅助选择/ae基因/轮回亲本/遗传背景回复率/连锁累赘Key words
marker-assisted selection/ amylose extender gene(ae)/ recurrent parent/ recovery ratio of background/ linkage drag分类
农业科技引用本文复制引用
乔岩,王汉宁,张成,杨芳..玉米胚乳突变基因ae连锁累赘的SSR分析[J].草业学报,2011,20(1):140-147,8.基金项目
国家高技术研究发展计划"863"计划项目(2004AA207140)资助. (2004AA207140)