林业科技开发2011，Vol.25Issue(1)：19-24,6.DOI:10.3969/j.issn.1000-8101.2011.01.005
金樱子与现代月季远缘杂交的生殖生物学特性
The characteristics of reproductive biology in the distant crosses between Rosa laevigata and Rosa hybridas
摘要
Abstract
Seeds are seldom obtained in the distant hybridization between R. hybridas and R. laevigata, which seriously influences utilization of excellent genes from R. laevigata. With an aim to reveal the factors leading to infertility in the crosses between modern rose varieties ( female parent) and R. laevigata ( male parent), we carried out the artificial orientation hybridization between diploid wild species in rosa-R. laevigata and four R. hybridas, which are ‘ Luwangte' 、‘ Gelin Jiaohong' 、‘ Belle de Londres' and ‘ Mount Shasta' , tested pollen viability of male parent, examined germination behavior of pollen grains on the stigmas after pollination, and observed embryo development. It was found that before the four R. hybridas pollinating, the pollen viability of male parent was respectively 8.0％ 、22.0％ 、63.7％ 、39.8％. No pollen grains germinated on the stigmas 2 h after pollination except for the stigmas of ‘ Belle de Londres' with 8.1, and respectively 17.6、21.9、11.7、12.0 pollen grains germinated on each stigma of the four varieties at 4 h after pollination; At 12 h after pollination, the number reached the highest value, which were 23.4、60.4、40.3、34.4 respectively; Subsequently, the number was quickly decreased, most pollen tubes were thin, short and curved, stayed on the stigmas' surface and failed to enter ovaries. In addition, during different time after pollination, average germinating rate on each stigma was increased first and then decreased. At 10 d after pollination, many globular embryo in ovaries began to degenerate, torpedo and cotyledonary embryos can be observed in the two crosses between R. laevigata and the two R. hybridas, which are ‘ Belle de Londres'and ‘ Mount Shasta' , but in the later phase, these embryos degenerated. These results suggest that the male pollen viability was not low, but 24 h after pollination, most germinated pollen grains on the stigmas were abnormal before fertilization,and degeneration of a large number of embryos after fertilization, which may be the main factors resulting in infertility in the cross.关键词
月季/远缘杂交/花粉活力/萌发形态/胚胎败育Key words
Rosa chinensis/ distant Hybridization/ pollen viability/ germination behavior/ embryo abortion引用本文复制引用
宋杰,王国良,金菁,刘冠楠,曾俊,杨阿芬..金樱子与现代月季远缘杂交的生殖生物学特性[J].林业科技开发,2011,25(1):19-24,6.基金项目
江苏省科技支撑计划(农业):"高抗浓香型大花藤本月季新品种选育"(BE2008400) （农业）
"十一五"国家科技支撑项目专题"优质高产月季新品种选育" (2006BAD01A1805). （2006BAD01A1805）
