山西医科大学学报2011，Vol.42Issue(1)：63-66,4.DOI:10.3969/J.ISSN.1007-6611.2011.01.016
正交设计优选玉米须多糖酶解提取工艺
Optimization of enzymolysis extraction technology of the polysaccharides in Corn Silk by orthogonal design
易延逵 1张璐 1刘莉 1郑飞 1翁立冬 1刘强1
作者信息
- 1. 南方医科大学中医药学院,广州,510515
- 折叠
摘要
Abstract
Objeaive To screen the best extraction conditions of the polysaccharides in Com Silk. Methods ①The extraction technology was optimized by the orthogonal test. ②The content of polysaccharides in Corn Silk was determined using different extraction methods with concentrated sulfuric acid-phenol colorimetric at 490 nm. Results The polysaccharide content showed no significant change by different enzymolysis extraction technologies , but the best hydrolysis time , hydrolysis temperature , enzyme concentration were different. The best cellulase enzymolysis conditions for the polysaccharides in Corn Silk were as follows : hydrolysis time 40 min , hydrolysis tempuature 80 ℃ ,enzyme concentration 1.O％ . The best caroid enzymolysis conditions for the polysaccharides in Com Silk were :hydrolysis time 120 min.hydrolysis temperature 40℃ ,enzyme concentration 1. 5％ . Conclusion This optimized technologies can be used to extract the polysaccharides from Com Silk , and aLso lay a foundation for the development of health care products.关键词
玉米须/多糖/提取/酶解/含量测定分类
医药卫生引用本文复制引用
易延逵,张璐,刘莉,郑飞,翁立冬,刘强..正交设计优选玉米须多糖酶解提取工艺 [J].山西医科大学学报,2011,42(1):63-66,4.
