物理化学学报2011，Vol.27Issue(1)：233-240,8.
氨基毗啶修饰碳纳米管的制备及其与辣根过氧化酶的相互作用
Preparation of Aminopyridine Grafted Carbon Nanotube and Its Interaction with Horseradish Peroxidase
摘要
Abstract
Carboxylic-functionalized multiwalled carbon nanotube (MWCNT-COOH) is obtained by oxidation with potassium bichromate and further modification by amide condensation afforded aminopyridine-grafted MWCNT (MWCNT-AP).The MWCNT-AP was characterized by Fourier transform infrared spectroscopy (FT-IR), proton nuclear magnetic resonance spectroscopy (1H-NMR) and X-ray photoelectron spectroscopy (XPS).Transmission electron microscopy (TEM) results suggest that MWCNTCOOH aggregates in ethanol and that MWCNT-AP is stable and well dispersed in solution.Horseradish peroxidase (HRP) physically adsorbed onto the surfaces of MWCNT-AP and MWCNT-COOH and the adsorption amounts were 187.5 and 153.0 μg·mg-1, respectively.UV-Vis spectra showed that the Soret band of HRP red-shifted markedly after adsorption onto MWCNT-AP or MWCNT-COOH indicating that the binding site of MWCNT-AP or MWCNT-COOH is near the heme pocket of HRP.Circular dichroism spectral results demonstrate that the secondary structure of HRP is influenced by MWCNT-AP.Enzyme-kinetic studies show that MWCNT-AP may adsorb HRP and its substrate 3,3’,5,5’-tetramethylbenzidine (TMB) effectively, and the maximum reaction rate (vmax) of HRP increases significantly after interaction with MWCNT-AP.关键词
碳纳米管/表面修饰/辣根过氧化酶/酶催化活性/氢键作用分类
化学引用本文复制引用
郝平,高云燕,欧植泽,李嫕,王中丽,王雪松..氨基毗啶修饰碳纳米管的制备及其与辣根过氧化酶的相互作用[J].物理化学学报,2011,27(1):233-240,8.基金项目
教育部留学回国人员科研启动基金(N9YK0003,N9YK0005),西北工业大学基础研究基金(NPU-FFR-JC200822)及西北工业大学翱翔之星项目(07XE0152)资助 （N9YK0003,N9YK0005）
