山西医科大学学报2011，Vol.42Issue(1)：29-32,4.DOI:10.3969/J.ISSN.1007-6611.2011.01.008
急性肺栓塞大鼠肺泡Ⅱ型上皮细胞形态及功能变化
Morphological and functional changes of type Ⅱ pneumonocytes in rats after acute pulmonary embolism
刘春萍 1崔俊玉 1陈杭薇 2陶然 3张力华 4陆慰萱5
作者信息
- 1. 北京军区总医院心内科,北京,100700
- 2. 北京军区总医院呼吸科
- 3. 北京军区总医院全军心理卫生指导中心
- 4. 盘锦市第四人民医院护理部
- 5. 北京协和医院呼吸科
- 折叠
摘要
Abstract
Objective To explore the changes of morphology and function of type Ⅱ pneumonocytes in rats with acute pulmonary embolism. Methods Sixteen male SD rats were randomized into control group(n =8) and embolism group( n =8). The rats were injected with medical gelfoam microsphe.res via jugular vein to induce PE model in embolism group.while in control group the rats were injected with saline instead of gelfoam granule soluiion. They were sacrificed at 24 h after establishing PE model. Pulmonary artery pressure , heart rate and respiratory rate were detected by right heart catheterization and artery blood gas was analyzed at 24 h. Bronchoalveolar lavage fluid( BALF) was collected for detecting the levels of phospholipid. Lung tissue was dyed with HE and the morphology of type Ⅱ pneumonocytes was observed under electric microscope. RT-PCR and Westem blot were used to study the expression of SPA mRNA and SPA protein in lung tissues. Results The mean pulmonary pressure and artery oxygen blood pressure were significantly different between control group and emholism group[ ( 14.2 ±4. 1) mmHg vs ( 26. 1 ±7. 52) mmHg,P ＜ 0. 05 ; ( 94. 1 ± 8. 8) mmHg vs ( 80. 5 ±5. 8) mmHg; ,P ＜ 0. 05 ] . The levels of phospholipid in BALF were ( 374. 17 ± 36. 55) mg/1 OO ml and 311. 67 ±t 48. 56) mg/l00 ml in control group and embolism group , respectively ( P ＜ 0. 05 ) _ The expression of SPA mRNA and protein was signif-icantly lower in embolism group than in control group ( 0. 83 ± 0. 33 vs 1. 43 ±0. 51 ,P＜0. 05 ;0. 44± 0. 18 v.s 1. 00 ±0. 00 , P ＜0. 05 ) . At 24 h, pulmonary arteries were seen emboliation with gelfoam under microscope. The microvillus of type Ⅱ pneumocytes reduced and desquamated under eletromicroscope,the mitochondria was hulged with fractured ridge and irregular empty lamina bodies, and the continuity of surfactant layer was interrupted. Conclusion The morphology and funccion of type Ⅱ pneumonocytes are changed significantly in acute pulmonary emholism rats,with decreased production of surfactant , which may play an important role in hypoxemia of pulmonary embolism.关键词
肺栓塞/大鼠/Ⅱ型肺泡上皮细胞分类
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刘春萍,崔俊玉,陈杭薇,陶然,张力华,陆慰萱..急性肺栓塞大鼠肺泡Ⅱ型上皮细胞形态及功能变化[J].山西医科大学学报,2011,42(1):29-32,4.
