华北农学报2011，Vol.26Issue(1)：31-36,6.
水稻T-DNA插入群体的建立及侧临序列的获得与分析
Construction of Rice T-DNA Insertion Population and the Acquisition and Analysis of Flanking Sequence
摘要
Abstract
In this study,the method of Agrobacterium-mediated transformation was used to transform GUS gene carried by plasmid 1301 into Nipponbare,and 5 200 rice T-DNA insertion population had been established. Studies showed that transgenic plants had been proved to be transformed by methods of PCR and Southern Blot,and 437 flanking sequence had been isolated by improving method of TAIL-PCR. By sequence homology analysis showed that there were 147 sequences containing only the vector sequence ,and 214 sequences containing T-DNA right border with the neighboring rice genome sequence. By comparison of the border sequences and public databases in the rice genome sequence,three mutants T-DNA insertion sites had been located in a particular rice chromosome. These results will provide important information for rice functional genomics research.关键词
T-DNA/插入突变/转基因水稻/TAIL-PCRKey words
T-DNA/ Insertional mutagenesis/ Transgenic rice/ TAIL-PCR分类
农业科技引用本文复制引用
魏进招,陈秋玲,裴忠有,孙守钧..水稻T-DNA插入群体的建立及侧临序列的获得与分析[J].华北农学报,2011,26(1):31-36,6.基金项目
天津市高等学校科技发展基金项目(20050905) （20050905）
