华北农学报2011，Vol.26Issue(1)：210-214,5.
转基因大豆对土壤氨氧化细菌的影响
Effect of Transgenic Soybean on Amount and Diversity of Ammonia-oxidizing Bacteria in Rhizospheric Soil
摘要
Abstract
The genetically modified (GM)crops are more and more concerned by public;meanwhile more and more GM crops are planted in fields globally. Besides the food safety, the ecological risks are also gained focus. In order to deeply understand the effect of transgenic soybean on diversity of rhizoperic ammonia-oxidizing bacteria in soil,DGGE-cloning and quantitative PCR were used. Both the DGGE-cloning and qPCR results showed that seasonal changes were observed throughout the soybean growth stages, indicating the impact of crop growth stage overweigh that of exogenous gene insertion and transgenic soybean has not diversely affect on rhizosphere ammonia-oxidizing bacteria abundance and community composition in soil.关键词
转基因大豆/根际土壤/氨氧化细菌/多样性/DGGE-cloning/定量PCRKey words
Transgenic soybean/Rhizospheric soil/Ammonia-oxidizing bacteria/Diversity/DGGE-cloning/qPCR分类
农业科技引用本文复制引用
赖欣,张永生,赵帅,杨殿林..转基因大豆对土壤氨氧化细菌的影响[J].华北农学报,2011,26(1):210-214,5.基金项目
中央级公益性科研院所基本科研业务费专项资金(2008-aepi-03) （2008-aepi-03）
