华北农学报2011，Vol.26Issue(1)：187-191,5.
BOX-PCR分子标记对补播紫花苜蓿共生根瘤菌田间竞争结瘤能力的研究
Study on Competitive Nodulation Ability of Rhizobia in Symbiosis with Reseeding Medicago sativa in Field Test by Using BOX-PCR Molecular Marker Method
摘要
Abstract
In this study,two rhizobial strains SX01 and HB02 were used to carry out the inoculation experiment with reseeding Medicago sativa Zhongmu no. 1 in You Yu county, Shanxi Province. In order to test the effect of rhizobial strains inoculation and screen high efficient rhizobia strains ,we used BOX-PCR Molecular Marker Method to Study the competitive nodulation ability of strain SXO1 and HB02 . The rhizobia strains reisolated from root nodules of alfalfa and inoculation stains were analyzed by BOX-PCR on meantime,the nodule occupancy of inoculation stains were detected by comparing BOX molecular fingerprint. The results showed that the nodule occupancy of two strains for SXO1 and HB02 were 46.7％ ,53.3％ respectively ,when the plant are harvest after the plant grew 60 days in the field ,this indicated that two rhizobia strains have high competitive abilities and can promote to use it in the field. In general ,the results suggested that BOX molecular marker method is an easy,rapid and accurate method for studying the competitive nodulation ability of inoculation of Rhizobia in soil.关键词
BOX-PCR/中苜一号/根瘤菌/占瘤率Key words
BOX-PCR/ Zhongmu no. 1/ Rhizobia/ Nodule occupancy分类
农业科技引用本文复制引用
肖猛,刘晓云,刘桂霞,戴燕燕,郭振国,郭晓叶,魏爽..BOX-PCR分子标记对补播紫花苜蓿共生根瘤菌田间竞争结瘤能力的研究[J].华北农学报,2011,26(1):187-191,5.基金项目
"十一五"国家科技支撑计划课题(2007BAD56B01) （2007BAD56B01）
