华北农学报2011，Vol.26Issue(1)：94-98,5.
高密度养殖下革胡子鲶生长激素mRNA表达的半定量分析
Semi-quantitative Analysis of Growth Hormone(GH) mRNA Expression of Clarias gariepinus in High Density Pond Culture
摘要
Abstract
Using β-actin mRNA expression quantity as an internal reference,the differences of growth hormone (GH) mRNA expression in pituitary of Clarias gariepinus in high density pond culture at different months old were analyzed with the employment of semi-quantitative RT-PCR method. The relative expression quantity of GH mRNA of individuals at 3.5,5 and 6.5 months old were 3. 612 - 5. 965,2. 929 - 4. 556 and 1. 360 - 1. 821 ,respectively, while the mean relative expressions were 4.346 ±0.656 0,3. 649 ±0.568 4 and 1. 575 ± 0. 135 5 separately for 3.5,5 and 6.5 months old. The variance analysis indicated that the significant differences reached 1％ in the relative expression level of the GH mRNA between any two months old. The Duncan's multiple comparison showed that the differences of GH mRNA expression between that at 3.5 and 6.5 months old as well as that at 5 and 6.5 months old were extremely significant,but there was no difference of GH mRNA expression between that at 3.5 and 5 months old.关键词
革胡子鲶/生长激素(GH)/半定量RT-PCRKey words
Clarias gariepinus/ Growth hormone/ Semi-quantitative RT-PCR分类
生物科学引用本文复制引用
王晓梅,戴伟,郭永军,陈承勋,李娜,张茂群..高密度养殖下革胡子鲶生长激素mRNA表达的半定量分析[J].华北农学报,2011,26(1):94-98,5.基金项目
天津市应用基础及前沿技术研究计划重点研究项目(08JCZDJC19000) （08JCZDJC19000）
