华北农学报2011，Vol.26Issue(1)：200-203,4.
与黄瓜抗炭疽病相关基因连锁的基因片段及SCAR标记
Resistance-related Gene Fragments and SCAR Marker for Identification of Cucumber Anthracnose Resistance
摘要
Abstract
In order to establish molecular marker-assisted selection system of cucumber anthracnose,with F1,F2 and BC1 population between a resistant parent(66)and susceptible parent(A18) ,we found that the resistance to anthracnose was controlled by a pair of recessive gene,and the susceptible was in the mode of incomplete dominant inheritance. A co-dominant AFLP marker was successfully converted into a simple, applied and co-dominant SCAR marker. Special marker SCEM178/172 was designed according to the sequence information of AFLP fragments, the marker could be useful in marker-assisted selection in cucumber breeding. The acquired SCAR marker had many advantages including fast, accurate, low cost, and free from an environmental influence, application of the acquired marker will enhance cucumber anthracnose disease resistant germplasm evaluation. 138 resistant germplasm were obtained from total 288.关键词
黄瓜/炭疽病/分子标记/SCARKey words
Cucumber/ Anthracnose/ Molecular marker/ SCAR分类
农业科技引用本文复制引用
王惠哲,管炜,李淑菊..与黄瓜抗炭疽病相关基因连锁的基因片段及SCAR标记[J].华北农学报,2011,26(1):200-203,4.基金项目
"十一五"国家科技支撑计划(2009BADB8B02) （2009BADB8B02）
天津市重点基金项目(09JCZDJC18900) （09JCZDJC18900）
