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6-BA浓度及基因型对大豆胚尖诱导丛生芽的影响

闫帆 孙昕 翟莹 李晓薇 王英 李景文 王庆钰

大豆科学2011,Vol.30Issue(1):29-32,4.
大豆科学2011,Vol.30Issue(1):29-32,4.

6-BA浓度及基因型对大豆胚尖诱导丛生芽的影响

Effect of Different 6-BA Concentration and Genotypes on Shoots Induced from Embryonic Tips

闫帆 1孙昕 1翟莹 1李晓薇 1王英 1李景文 1王庆钰1

作者信息

  • 1. 吉林大学植物科学学院,吉林,长春,130062
  • 折叠

摘要

Abstract

In order to establish an efficient embryonic tip regeneration system for soybean (Glycine max L. Merr. ), five soybean cultivars, including Jilin 35, were used as explants to study the effects of 6-BA and genotypes on the numbers and induction rate of adventitious shoots. The endurance of the induced shoots of Jilin 35 to Hygromycin B was also studied. The results showed that Jilin 35 was fit for soybean embryonic tip regeneration system according to the numbers and inductivity of adventitious shoots. The concentration of 3.0 mg· L-1 Hygromycin B was advised for resistance screening in embryonic tip regeneration system.

关键词

大豆/胚尖/6-BA/基因型

Key words

Soybean/ Embryonic tip/ 6-BA/ Genotype

分类

农业科技

引用本文复制引用

闫帆,孙昕,翟莹,李晓薇,王英,李景文,王庆钰..6-BA浓度及基因型对大豆胚尖诱导丛生芽的影响[J].大豆科学,2011,30(1):29-32,4.

基金项目

转基因生物新品种培育重大专项资助项目(2008ZX08004-003) (2008ZX08004-003)

国家自然科学基金面上资助项目(30971808) (30971808)

吉林省科技发展计划重点资助项目(20080204) (20080204)

长春市科技局国际科技合作资助项目(08GH10) (08GH10)

"211工程"三期重点学科建设资助项目. ()

大豆科学

OA北大核心CSCDCSTPCD

1000-9841

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