分析化学2011，Vol.39Issue(1)：103-106,4.
镧掺杂二氧化钛/沸石微柱在线预富集-分光光度法测定溶菌酶含量
Spectrophotometric Determination of Lysozyme by On-line Preconcentration with A Microcolumn Containing La3+-TiO2-Zeolite
摘要
Abstract
A spectrophotometric method for the determination of lysozyme was established based on the on-line preconcentration with a La3+-TiOz-zeolite microcolumn. Effects of operation parameters on the extraction efficiency were optimized as follows: methanol as the eluent with a flow rate of 2.0 mL/min, sample pH of 5.0, sample flow rate of 1.2 mL/min, sample volume of 1.2 mL, and detection wavelength of 280 nm. The proposed method was employed for the determination of lysozyme in egg white, honey, and wine samples. The relative standard deviations of intra-day and interday were determined as 2. 1 o％ and 2. 8％, respectively. The recoveries were higher than 90％. The method is simple, rapid, sensitive.关键词
镧掺杂二氧化钛/沸石/微柱/分光光度法/溶菌酶Key words
Lanthanum(Ⅲ)-titamium oxide-zeolite/ Microcolumn/ Spectrophotometry/ Lysozyme引用本文复制引用
田苗苗,苏日艳,贾琼,鲍长利,权新军..镧掺杂二氧化钛/沸石微柱在线预富集-分光光度法测定溶菌酶含量[J].分析化学,2011,39(1):103-106,4.基金项目
本文系中国科学院长春应用化学研究所稀土资源利用国家重点实验室开放基金(2008)项目 （2008）
