法医学杂志2011，Vol.27Issue(1)：9-12,21,5.DOI:10.3969/j.issn.1004-5619.2011.01.003
猪眼玻璃体液钾离子、次黄嘌呤浓度变化与PMI推断
Concentration Changes of Potassium and Hypoxanthine in Vitreous Humor of Swine and Its Application to Postmortem Interval Estimation
方超 1王松才 2孙立敏 2张小婷 2龙伟清 3竞花兰1
作者信息
- 1. 中山大学,中山医学院法医病理教研室,广东,广州,510080
- 2. 广州市刑事科学技术研究所,广东,广州,510030
- 3. 中山大学,附属第一医院检验科,广东,广州,510080
- 折叠
摘要
Abstract
Objective To investigate the relationship between postmortem interval (PMI) and concentration changes of components in swine vitreous humor. Methods Ninety-six porcine eyes from swine dying from acute massive hemorrhage, being randomly divided into 24 groups, were stored in dark situation, at temperature of (15+2)℃ and humidity of(50+5)％ for 2-96 hours separately. The vitreous humor was collected.Concentrations of K+, Na+, CI- and hypoxanthine(Hx) were analyzed by automatic biochemical analyzer and ultra performance liquid chromatograph (UPLC). The data were statistically analyzed by SPSS software.Results Linear regression analysis showed that concentrations of vitreous K+ and Hx were positively correlated with PMI(R2-0.767 and R2=0.793, respectively). Binary linear regression showed a higher correlation for K＋ and Hx with PMI estimation (R2=0.866). PMI was not significantly correlated with vitreous Na+ and C1- concentrations. Conclusion Vitreous K+ and Hx concentrations can be used as the objective markers for PMI estimation. The binary linear regression functions of vitreous K+ and Hx concentrations with PMI are more accurate for estimating the PMI.关键词
法医病理学/次黄嘌呤/钾/玻璃体液/死亡时间/猪Key words
forensic pathology/ hypoxanthine/ potassium/ vitreous humor/ postmortem interval/ swine分类
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方超,王松才,孙立敏,张小婷,龙伟清,竞花兰..猪眼玻璃体液钾离子、次黄嘌呤浓度变化与PMI推断[J].法医学杂志,2011,27(1):9-12,21,5.
