法医学杂志2011，Vol.27Issue(1)：33-35,42,4.DOI:10.3969/j.issn.1004-5619.2011.01.009
激光捕获显微切割技术用于分离混合斑中精子细胞
Isolating Sperm Cells by Laser Capture Microdissection Technique from Mixture Sample
刘芳 1王静 2俞丽娟 1郭剑章 1高俊薇 2焦章平 2唐晖 2刘雅诚2
作者信息
- 1. 山西医科大学,法医学院,山西,太原,030001
- 2. 北京市公安局,法医检验鉴定中心,北京,100192
- 折叠
摘要
Abstract
Objective To assess the application value of laser capture microdissection (LCM) technique for isolating a small number of sperm cells from mixture sample. Methods Mixture samples were prepared with sperm cells and vaginal epithelia at different concentrations. Both LCM technique and the differential lysis method were employed to obtain sperm cells from the mixture samples, and DNA was extracted by magnetic beads method. STR genotyping was determined using IdentifilerTM kit. Results The successful STR genotype rate of sperm cells isolated from mixture samples with LCM technique was 92.86％(13/14). The rate of differential lysis method was 7.14％(1/14). The successful rates between the two methods were statistically different (P＜0.05). Conclusion LCM technique can effectively exclude the interference of female cell component and isolate a small number of sperm cells to obtain a single male STR genotyping. LCM technique is obviously better than the differential lysis method.关键词
法医遗传学/精子细胞/混合斑/激光捕获显微切割技术Key words
forensic genetics/ sperm cell/ mixed stain/ laser capture microdissection(LCM)分类
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刘芳,王静,俞丽娟,郭剑章,高俊薇,焦章平,唐晖,刘雅诚..激光捕获显微切割技术用于分离混合斑中精子细胞[J].法医学杂志,2011,27(1):33-35,42,4.
