分析化学2011，Vol.39Issue(1)：1-6,6.
人肝细胞中砷代谢产物分析表征研究
Characteristics of Arsenic Metabolites in Human Liver Hepatocytes
摘要
Abstract
Hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) was applied to the determination of arsenic metabolites in Chang liver cells after arsenic incubation. Two unknown arsenicals were found, one of which was speculated as methylarsonous acid (MMA Ⅲ ), and further characterized using standard addition method using synthesized MMAⅢ standard. Quantitative analysis results showed that monomethylarsenicals, dimethylarsenicals and inorganic arsenicals in hepatocytes after arsenic incubation were increasing with the increase of initial incubated arsenic concentration while the methylation rate decreasing with the increase of initial incubated arsenic concentration, which indicated that high concentration of arsenic has the inhibition to the ability of arsenic methylation of Chang liver cells.关键词
高效液相色谱/电感耦合等离子体质谱/飞行时间质谱/Chang肝细胞/砷形态/一甲基亚胂酸Key words
High performance liquid chromatography-inductively coupled plasma-mass spectrometry/High performance liquid chromatography-time of flight-mass spectrometry/ Chang liver cells/ Arsenic metabolites/ Methylarsonous acid引用本文复制引用
曹煊,余晶晶,高扬,孙继昌,刘岩,徐苑苑,孙贵范,王小如..人肝细胞中砷代谢产物分析表征研究[J].分析化学,2011,39(1):1-6,6.基金项目
本文系国家自然科学基金(No.20675021),山东省科学院博士基金(No.Y09-3)资助项目 （No.20675021）
