法医学杂志2011，Vol.27Issue(1)：5-8,4.DOI:10.3969/j.issn.1004-5619.2011.01.002
大鼠死后脑、心肌和肾组织β-actin mRNA的降解与早期死亡时间的关系
Estimation of Early Postmortem Interval Using β-actin mRNA in Rat's Brain,Heart and Kidney
刘岳霖 1马开军 2李文灿 1徐红梅 1薛爱民 1沈忆文 1周月琴 1赵子琴1
作者信息
- 1. 复旦大学,上海医学院法医学系,上海,200032
- 2. 上海市公安局,物证鉴定中心,上海,200083
- 折叠
摘要
Abstract
Objective To explore the relationship between β-actin mRNA degradation in SD rat's brain,heart and kidney and early postmortem interval (PMI) in order to find new markers for estimating early PMI. Methods Rats were sacrificed and kept in the place at a temperature of 20℃. The total RNA were extracted from the brain, heart and kidney at different PMI points. Real time RT-PCR was applied to determine β-actin mRNA levels in total RNA and the results were given in the form of Ct values. Linear relationships between PMI and Ct values were obtained and the functions of linear regression were established. Results The great decrease of β-actin mRNA level were observed in the three organs. The degradation rate was obviously higher in 24 hours after death in the heart and kidney. However, there were no significant changes in the brain. The changes of Ct values and PMI showed a good linear relationship.Conclusion β-actin mRNA in rat's brain, heart and kidney degrades obviously after death and can be used for estimating early PMI by its degradation rules.关键词
法医病理学/聚合酶链反应/脑/心肌/肾/β-actin mRNA/死亡时间/大鼠Key words
forensic pathology/ polymerase chain reaction/ brain/ myocardium/ kidney/ β-actin mRNA/ postmortem interval/ rats分类
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刘岳霖,马开军,李文灿,徐红梅,薛爱民,沈忆文,周月琴,赵子琴..大鼠死后脑、心肌和肾组织β-actin mRNA的降解与早期死亡时间的关系[J].法医学杂志,2011,27(1):5-8,4.
