安徽农业科学2011，Vol.39Issue(1)：447-450,4.
1株高效聚磷节杆菌的分离鉴定及其聚磷特性研究
Study on Isolation, Identification and Phosphate Accumulating Capacity of Arthrobacter sp.
陈燕 1杨小龙 1曹郁生1
作者信息
- 1. 南昌大学食品科学与技术国家重点实验室,南昌大学中德联合研究院,江西南昌,330047
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摘要
Abstract
[ Objective ] The aim was to reduce the content of phosphorus in water so as to control the water eutrophication, and select phosphorus accumulating bacteria for biological phosphorus removal. [ Method ] With the phosphorus accumulating capacity as the main index, the bacteria were isolated and selected, their culture conditions were optimized so as to improve their phosphorus accumulating capacity. Then the isolated high-efficient phosphorus accumulating bacteria were identified based on the biochemical characteristics and molecular biological analysis. [ Result ] Among the nine effective phosphorus accumulating bacteria isolated from sludge and sewage, J-12 showed the highest phosphorus accumulating capacity. 16S rDNA sequence analysis indicated that the homology of J-12 and Arthrobacter sp. reached 99％, taking into account its growth characteristics and physiological and chemical properties, it was preliminarily determined that J-12 belonged to one species of Arthrobacter sp. , the Arthrobacter sp. with high-efficient phosphorus accumulating capacity hasnt been reported. The study showed that, after 10 h of culture under 32 ℃, 7.0 of pH and 4 ml addition of trace element solution, J-12 could reduce the total phosphorus content in the synthetic water from 20. 0 mg/L to 0. 1 mg/L, its phosphorus accumulating rate could reach above 95％. [ Conclusion] The selected arthrobacter with high-efficient phosphorus accumulating capacity had potential application prospect in sewage treatment and aquaculture.关键词
节杆菌/聚磷特性/同源分析/鉴定Key words
Arthrobacter/ Phosphorus accumulating property/ Homology analysis/ Identification分类
生物科学引用本文复制引用
陈燕,杨小龙,曹郁生..1株高效聚磷节杆菌的分离鉴定及其聚磷特性研究[J].安徽农业科学,2011,39(1):447-450,4.
