福建农林大学学报（自然科学版）2011，Vol.40Issue(1)：54-57,4.
悬钩子皮下盘菌种内遗传多样性的ITS序列分析
ITS sequence analysis of intraspecific genetic diversity of Hypoderma rubi
摘要
Abstract
Fresh materials of Hypoderma rubi collected from Anhui Province were isolated by tissue isolation technique, and 12 pure strains were obtained. DNAs of all the strains were extracted from the dry mycelia, rDNA-ITS regions were amplified by PCR and then sequenced. The ITS sequence analysis results showed that the length range of ITS sequences of H. rubi was from 443 to456 bp,and the percentage of G + C contents of the ITS sequences varied from 48.7％ to 51.5％. There were obvious variations in the ITSsequences of different strains and showed abundant genetic diversity among them. A phylogenetic tree was constructed by neighbourjoining method using MEGA 4.1. The results indicated that the intraspecific genetic diversity of H. rubi was related to hosts obviously, and strains from woody plants and herbaceous plants probably were two distant relationship groups. In addition, the intraspecific genetic differences of H. rubi were independent of parasitic sites and sample collection area.关键词
皮下盘菌属/模式种/rDNA-ITS序列/遗传多样性/寄主Key words
Hypoderma/ type species/ rDNA-ITS sequence/ genetic diversity/ host分类
农业科技引用本文复制引用
刘芳,林英任,王士娟,高小明,陈莉,贾国军..悬钩子皮下盘菌种内遗传多样性的ITS序列分析[J].福建农林大学学报（自然科学版）,2011,40(1):54-57,4.基金项目
国家自然科学基金(30870014,30770006) （30870014,30770006）
教育部高校博士点专项科研基金(20070364002) （20070364002）
安徽省自然科学基金(070411005). （070411005）
