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基于形态及SRAP标记的辣椒资源遗传多样性及亲缘关系比较

许先松 刘志钦 林晓丹 牟少亮 官德义 何水林

福建农林大学学报(自然科学版)2011,Vol.40Issue(1):48-53,6.
福建农林大学学报(自然科学版)2011,Vol.40Issue(1):48-53,6.

基于形态及SRAP标记的辣椒资源遗传多样性及亲缘关系比较

Genetic diversity and relationship analysis of pepper germplasm resources based on phenotype traits and SRAP molecular markers

许先松 1刘志钦 1林晓丹 1牟少亮 1官德义 2何水林1

作者信息

  • 1. 福建农林大学生命科学学院
  • 2. 福建农林大学作物科学学院,福建,福州,350002
  • 折叠

摘要

Abstract

Germplasm resources is important material basis for crop genetic improvement, the genetic relationship and genetic diversity study of pepper germplasms is important base of pepper genetic improvement. In this paper, the genetic relationship and genetic diversity were studied thorough the evaluation of phenotypic traits as well as sequence-related amplified polymorphism (SRAP) molecular markers, the main results were as follows: Based on the cluster analysis of fruit traits, color of leaves and other morphologicai traits, the pepper germplasms in the experiment were clustered into 4 groups when the index of genetic similarity is 0.69. The 4 groups are CatnicumannuumL. var. conoides , Capsicum annuum L. var. conoides bailey , C apsicum annuum L var. longum baily and Capsicum annuun L. var. cerasiforme baily. Using 17 pairs of SRAP primers, genetic diversity and genetic relationship among 72 germplasms were studied, a total of 182 bands were acquired by PCR, about 118 of them showed diversity in length. The genetic similarity index among the 72 pepper gennplasms varied from 0.56 to 0.91, and the 72 gennplasms were divided into 8 groups when the genetic similarity index is 0.737. A deviation was found between the genetic diversity and genetic relationship by SRAP marker and morphological marker in this study, and a more abundance and stable genetic diversity was found by SRAP marker on the pepper germplasms, suggesting its value in the genetic diversity and genetic relationship study of crops such as poppers.

关键词

辣椒/SRAP/表型/聚类分析/种质资源

Key words

pepper/ SRAP/ morphological/ cluster analysis/ germplasm resources

分类

农业科技

引用本文复制引用

许先松,刘志钦,林晓丹,牟少亮,官德义,何水林..基于形态及SRAP标记的辣椒资源遗传多样性及亲缘关系比较[J].福建农林大学学报(自然科学版),2011,40(1):48-53,6.

基金项目

福建省自然科学基金资助项目(2008J0003) (2008J0003)

福建省科技厅重点项目(2008N0099). (2008N0099)

福建农林大学学报(自然科学版)

OA北大核心CSCDCSTPCD

1671-5470

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