福建农林大学学报（自然科学版）2011，Vol.40Issue(1)：69-73,5.
一株水产地衣芽孢杆菌的鉴定及培养基优化
Identification of an aquatic Bacillus licheniformis CJ001 and optimization of the culture media
摘要
Abstract
Strain CJ001 was isolated and selected from sediment samples of breed aquatics pond in Wuhan. Based on its major morphologic, physiology-biochemistry characteristics and 16S rDNA sequence analysis, strain CJ001 was identified as Bacilluslicheniformis. The strain could degrade the chemical oxygen demand (COD) in aquacultural water effectively, especially at the time of 48 h ( with 86.8％ degradation rote) or under the temperature of 30 ℃ ( with 85.7％ degradation rate). According to the results of single-factor orthogonal test for culture optimization, the orthogonal experiment with 5 factors (brown sugar, com powder, bean, carbamide and NaH2PO4) and 4 levels was then carried out. The optimal medium was as follows: brown sugar 3％, compowder 1％,bean 3％, carbamide 0.25％ and NaH2PO4 0.15％, respectively. Under the condition, 11.15 × 109 cfu bacteria per milliliter were obtained.关键词
地衣芽孢杆菌/水产养殖/化学需氧量/培养基优化/正交试验Key words
Bacillus licheniformis/ aquaculture/ chemical oxygen demand/ media optimization/ orthogonal experiment分类
生物科学引用本文复制引用
袁科平,邹世平,李谷,何力,周群兰..一株水产地衣芽孢杆菌的鉴定及培养基优化[J].福建农林大学学报（自然科学版）,2011,40(1):69-73,5.基金项目
中国水产科学研究院基本科研业务费资助项目(2007-008) （2007-008）
热带亚热带鱼类选育与养殖重点开放实验室开放课题. （）
