高等学校化学学报2011，Vol.32Issue(1)：38-43,6.
蟾酥急性毒性的代谢组学研究
Metabonomics Study on the Acute Toxicity of Toad Venom
摘要
Abstract
The metabonomics method coupled with electrocardiogram were applied to the study on the acute toxicity of toad venom. The profiles of serum samples were obtained with ultra performance liquid chromatography coupled with time-of-flight mass spectrometry(UPLC/TOF-MS). The data were firstly processed with the software Markerlynx and then analyzed with the principal components analysis (PCA) and orthogonal partial least squares discriminate analysis(OPLS-DA). The potential biomarkers were screened out according to the VIP( variable importance in projection) value and identified with t-fit, database and corresponding reference standards. The metabolites of significant intergroup differences were finally found to be li-pids related to myocardial energy metabolism. According to the biological functions of the obtained potential biomarkers, it was deduced that the heart injury may be induced by disturbing lipid metabolism through hampering the re-acylation of free fatty acid or activating protein kinase pathway. This study provides a new approach to discover the underlying mechanism of toad venom toxicity.关键词
蟾酥/急性毒性/代谢组学/超高效液相色谱-飞行时间质谱/脂质代谢Key words
Toad venom/ Acute toxicity/ Metabonomics/ UPLC/TOF-MS/ Lipid metabolism分类
化学化工引用本文复制引用
梁晓萍,张政,胡坪,钟宏福,葛朝晖,邹婷婷,梁琼麟,王义明,罗国安..蟾酥急性毒性的代谢组学研究[J].高等学校化学学报,2011,32(1):38-43,6.基金项目
国家"新药创制"重大专项基金(批准号:2008ZX09202,2009ZX09311-001)和国家"十一五"科技支撑计划项目(批准号:2006BAI08B04-01)资助. （批准号:2008ZX09202,2009ZX09311-001）
