高等学校化学学报2011，Vol.32Issue(1)：67-73,7.
微生物酶催化制备人参皂苷20(S)-Rg2,20(S)-Rh1和20(S)-PPT
Enzyme-catalyzed Preparation of 20 (S)-Ginsenoside Rg2,20(S)-Ginsenoside Rh1 and 20 (S) -PPT
摘要
Abstract
The prosapogenins of ginsenosides have significant phamacological activities such as antitumor and antimetastatic effects. Owing to low or non-existent content of the prosapogenins in normal ginseng, it is necessary and meaningful to prepare more active prosapogenin by degradation of major ginsenosides. In this paper,ginsenosides Re and Rgl were hydrolyzed by crude enzyme of strain Microbacterium esteraromaticum GS514 and the resultant's structures were determined by 1 H NMR and 13C NMR analyses. The experimental results show that the reaction of ginsenosides Re and Rgl with crude enzymes is affected by NaCl treatment. If ginsenosides Re and Rgl reacted with crude enzymes directly, ginsenoside Re could not be converted into other ginsenosides and ginsenoside Rgl was converted into ginsenoside Fl through selective hydrolysis of β-D-glucopyranosyl moiety connected with C6. But if the reaction was carried out in the presence of NaCl,ginsenoside Re converted into 20(S)-ginsenoside Rg2, ginsenoside Rgl into 20 (S)-ginsenoside Rhl and 20(S)-protopanaxatriol through selective hydrolysis of β-D-glucopyranosyl moiety connected with C20. These results indicate that C20 β-D-glucopyranoside enzyme is activated by NaC1, and it has significant effect on the production of different secondary ginsenosides by high regioselectivity and stereoselectivity.关键词
酶催化制备/20(S)-人参皂苷Rg2/20(S)-人参皂苷Rh1/20(S)-原人参三醇Key words
Enzyme-catalyzed preparation/ 20(S)-ginsenoside Rg2/ 20(S)-ginsenoside Rhl/ 20(S)-protopanaxatriol分类
化学化工引用本文复制引用
成乐琴,金瑜真,梁德春..微生物酶催化制备人参皂苷20(S)-Rg2,20(S)-Rh1和20(S)-PPT[J].高等学校化学学报,2011,32(1):67-73,7.基金项目
韩国科技部21世纪前沿研发计划植物多样性研究中心(批准号:PF0622200)资助. （批准号:PF0622200）
