高等学校化学学报2011，Vol.32Issue(1)：62-66,5.
罗丹明类荧光探针的合成及对铜离子的检测
Synthesis of Fluorescent Probe Based on Rhodamine and Its Appalication in the Determination for Cu2+
摘要
Abstract
In this paper, Cu2+ fluorescence enhancement probe 3',6'-bis ( diethylamino)-2-(N-ethylideneamino) spiro [ isoindoline-1,9'-xanthen ]-3-one (RA) based on rhodamine B was synthesized. It was found that the fluorescence intensity of RA increased with the addition of Cu2 + in acetonitrile/water( 1: 1, volume ratio) solution. The maximum absorption wavelength was 548 nm. The maximum emission wavelength was 571 nm. The addition of Cu2+ ion led to an obvious color change to pink as well as significantly enhanced fluorescence, while other common metal ions such as Na + , K + , Mg2+ , Ca2+ , Mn2+ , Cd2+ , Cr3+ , Co2+ , Ni2+ ,Ag+ , Pb2 +, Zn2 + , Fe3 + and Hg2 + induced no or much smaller color/fluorescent spectral changes. The selective fluorescence enhancement of RA attributed to the spirolactam ring being opened upon the addition of Cu2+to RA, and a highly delocalized conjugated structure of RA was formed. The sensor was useful for measuring Cu2+ at concentrations ranging from 6.5 x 10-8 to 2. 9 x 10-6 moi/L with a detection limit of 5.0 x 10-8 mol/L. The recognition of Cu2+ was not reversible, and compound RA was pH-insensitive Cu2+ fluorescent probe,which would detect Cu2+ effectively in a relatively wide pH range(4.1-10.5).关键词
荧光探针/Cu2+/选择性/pH值Key words
Fluorescent probe/ Cu2 +/ Selectivity/ pH value分类
化学化工引用本文复制引用
袁跃华,冯锋,田茂忠,孟双明,白云峰..罗丹明类荧光探针的合成及对铜离子的检测[J].高等学校化学学报,2011,32(1):62-66,5.基金项目
国家自然科学基金(批准号:20575057)、山西省自然科学基金(批准号:2009011015-1)、山西省回国留学人员科研项目(批准号:20091180)和山西大同大学科学基金(批准号:2009K4)资助. （批准号:20575057）
