广东药学院学报2011，Vol.27Issue(1)：48-50,3.DOI:10.3969/j.issn.1006-8783.2011.01.012
RP-HPLC内标法测定大鼠血浆中甘草次酸的浓度
Determination of glycyrrhetinic acid in rat plasma by RP-HPLC
摘要
Abstract
Objective To develop a HPLC assay for the determination of glycyrrhetinic acid in rat plasma.Methods Analytical column was Diamonsil C18 column(4. 6 mm × 25 cm,5 μm) at 30 ℃ with the mobile phase consisted of methanol,water and acetic acid ( 86∶13. 6∶0. 4). The flow rate was 1. 0 mL · min -1 , and the UV detection was set at 250 nm. The sample size was 20 μL,and testosterone propionate was used as the internal standard. Results The linear calibration curve was obtained in the concentration range of 0.1 - 3. 0 mg · L-1(r = 0. 999 9). The lower limit of qualification was 0. 1 mg · L-1. The intra-day and inter-day RSD were less than 6％ . Conclusion The method is simple , rapid and suitable for the determination of glycyrrhetinic acid in rat plasma and the study of pharmacokinetics.关键词
甘草次酸/高效液相色谱法/血药浓度/丙酸睾酮分类
医药卫生引用本文复制引用
郭波红,林绿萍,林德晖,程怡..RP-HPLC内标法测定大鼠血浆中甘草次酸的浓度[J].广东药学院学报,2011,27(1):48-50,3.基金项目
国家自然科学基金(30772790) （30772790）
