棉花学报2011，Vol.23Issue(1)：52-57,6.
人工模拟"庇护所"和"非庇护所"条件下棉铃虫对 Bt 棉的抗性演变
Studies on Resistance Evolution of Cotton Bollworm to Bt Transgenic Cotton in Artificial Simulating ＂Refuges＂ and ＂Non-refuges＂ Conditions
摘要
Abstract
In order to rind out the resistance evolution of Helicoverpa armigera(Hubner) to Bt cotton, ＂refuges＂ and ＂non-refuges＂conditions were simulated. Laboratory screening was conducted for obtaining the resistant strains of inbred and hybrid line feeding on the artificial diet which contained cotton seeds powder from Bt hybrid CCRI 29. The results showed that: screening for 11 generations, the mortality rates of inbred strain and hybrid strain of H. armigera larva decreased, larval weight and larval length increased, the larval duration shortened, pupation rate and emergence rate increased. Contrast with the hybrid strain, the inbred strain adapted faster to Bt transgenic cotton. Screening for 1 1 generations, using different resistant strains of cotton bollworm for transgenic insect-resistance assessment, results indicated that the resistance grade of phase-sensitive strains determination of four insect-resistant transgenic cotton cultivars did not change, and resistance grade of three transgenic varieties to self-bred lines from CCRI 29, Nankang-3, and Kemian-1 decreased by one. The resistance ratio of self-strain and hybrid strain to Bt preparations was 9.3876 and 2.5215, respectively. According to the preliminary results of this study, the policy of ＂refuges＂ to delay the resistance of cotton bollworm to Bt transgenic cotton is feasible.关键词
棉铃虫/转Bt基因棉/抗性筛选Key words
cotton bollworm/ Bt transgenic cotton/ resistance screening分类
生物科学引用本文复制引用
肖留斌,孙厚俊,柏立新..人工模拟"庇护所"和"非庇护所"条件下棉铃虫对 Bt 棉的抗性演变[J].棉花学报,2011,23(1):52-57,6.基金项目
国家"十一五"重大科技支撑计划(2006BAD08A07) （2006BAD08A07）
国家转基因项目--转基因棉花环境安全评价技术(2008ZX08011-002) （2008ZX08011-002）
现代农业产业技术体系建设专项资金 （）
