棉花学报2011，Vol.23Issue(1)：22-27,6.
棉花多重 PCR 技术及其对杂交棉纯度鉴定的初步研究
Preliminary Study on Multiplex PCR Technique and Its Application in Hybrid Cotton Seed Purity Test
摘要
Abstract
A series of stable polymorphism SSR primers in Xiangzamian series cultivars were used to construct various primer combinations according to the difference of their amplified band's size for multiplex PCR amplification of cotton.Eighty percent of double PCR amplified normally based on the principle of amplified fragments size difference under the same conditions as their single PCR reactions.Besides, triplex and quadruple PCR were also amplified normally using primers of double PCR with good results.So we proposed a simplified protocol of multiplex PCR assay in cotton.The multiplex PCR technology was used to detect the purity of hybrid cotton seeds which clearly identified impurity and blend of female parent seeds and also other hybrid cotton seeds.It can be used as a reference for the rapid and accurate identification of cotton hybrids.关键词
棉花/SSR/多重PCR/纯度检测Key words
cotton/ SSR/ multiplex PCR/ identification of seed purity分类
农业科技引用本文复制引用
陈浩东,刘方,王为,肖才升,李庠,王琳,李育强,王坤波..棉花多重 PCR 技术及其对杂交棉纯度鉴定的初步研究[J].棉花学报,2011,23(1):22-27,6.基金项目
国家863计划(2009AA101104),国家科技支撑计划(2006BAB01A05),国家自然科学基金(30900911) （2009AA101104）
