广东药学院学报2011，Vol.27Issue(1)：44-47,4.DOI:10.3969/j.issn.1006-8783.2011.01.011
DM130型大孔树脂分离纯化野菊花总黄酮的工艺研究
Purification of the total flavonoids from Chrysanthemum indicum with DM130 macroporous resin
刘承萍 1高峰 1曹骋 1曾元儿1
作者信息
- 1. 广州中医药大学中药学院,广东,广州,510006
- 折叠
摘要
Abstract
Objective To investigate the conditions of purifying total flavonoids from Chrysanthemum indicum with macroporous adsorption resin. Methods Static and dynamic tests of macroporous resin were employed to study on the purification effect and influencing factors with the recovery rate of total flavonoids as index.Results The purification conditions of DM130 resin were as follows : the concentration of total flavonoids was 2.78 mg/mL at pH3 -4,80％ alcohol eluting for 5 times as much as the resin volume, and the elution velocity was 2 mL/min. The recovery of total flavonoids was 79. 19％ , and the purification rate was 37. 31％. Conclusion The optimum technology is stable and feasible, and DM130 resin is suitable for separating and purifying total flavonoids from Chrysanthemum indicum.关键词
野菊花/总黄酮/大孔树脂/纯化分类
中医学引用本文复制引用
刘承萍,高峰,曹骋,曾元儿..DM130型大孔树脂分离纯化野菊花总黄酮的工艺研究[J].广东药学院学报,2011,27(1):44-47,4.
