广东药学院学报2011，Vol.27Issue(1)：51-53,3.DOI:10.3969/j.issn.1006-8783.2011.01.013
HPLC法测定不同产地党参药材中党参炔苷的含量
Determination of lobetyolin in Radix Codonopsis from different origins by HPLC
时军 1马方励 2廖华卫 1梁培华1
作者信息
- 1. 广东药学院,中药学院,广东,广州,510006
- 2. 无限极(中国)有限公司,广东,广州,510665
- 折叠
摘要
Abstract
Objective To determine the content of lobetyolin in Radix Codonopsis from different regions by HPLC. Methods HPLC was carried out on Diamonsil C18 column(250 mm ×4. 6 mm,5 μm) with the mobile phase acetonitrile-water(25∶75 ) and the UV detector was set at 268 nm. The injection volume was 20 μL.Results The calibration curve was linear in the range of 0. 008 5 - 0. 425 mg/mL( r =0. 999 5 ) ,and the average recovery rate was 101.4％ . The contents of lobetyolin in Radix Codonopsis from 9 origins were varied between 0. 187 mg/g and 1. 174 mg/g. Conclusion The method is simple, rapid and reproducible, which can be used for the quality control of Radix Codonopsis.关键词
党参/党参炔苷/高效液相色谱法分类
医药卫生引用本文复制引用
时军,马方励,廖华卫,梁培华..HPLC法测定不同产地党参药材中党参炔苷的含量[J].广东药学院学报,2011,27(1):51-53,3.
