广东药学院学报2011，Vol.27Issue(1)：5-9,5.DOI:10.3969/j.issn.1006-8783.2011.01.002
巴戟甲素表观油水分配系数和血清蛋白结合率的测定
Determination of the apparent oil-water partition coefficient and serum protein binding rate of Bajijiasu
摘要
Abstract
Objective To determine the apparent oil-water partition coefficient and serum protein binding rate of Bajijiasu which might support for its pharmacokinetics research in vivo. Methods HPLC-ELSD was used for the assay of Bajijiasu , and the partition coefficients were determined by shaking flask method. The ultrafiltration method was employed to obtain the serum protein binding rates. Results The apparent oilwater partition coefficient in octanol/water system was 0. 052 5 ( logpapp = - 1. 28) and 0. 050 7 ( logpapp = - 1. 295) in octanol/phosphate buffer ( pH = 6. 8) system. The serum protein binding rates of Bajijiasu with BSA, HSA and rat serum protein were 42. 15％ 、40. 67％ 、30. 89％ , respectively. Conclusion Bajijiasu was a hydrotropic substance. The partition coefficients in octanol/water system is identical with octanol/ phosphate buffer ( pH = 6. 8) system. The serum protein binding rate of Bajijiasu with BSA 、HAS was significantly higher than other rat serum protein, which showed that Bajijiasu may have a kind of specific binding with albumin in serum.关键词
巴戟甲素/表观油水分配系数/蛋白结合率分类
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陈永刚,林励,吴金虎..巴戟甲素表观油水分配系数和血清蛋白结合率的测定[J].广东药学院学报,2011,27(1):5-9,5.基金项目
海南省重点科技项目(090603) （090603）
