土壤学报2011，Vol.48Issue(1)：132-140,9.
不同氮效率水稻生育后期氮代谢酶活性的变化特征
VARIATION OF ACTIVITY OF N METABOLIZING ENZYMES IN RICE PLANTS DIFFERENT IN N USE EFFICIENCY AT THEIR LATE GROWTH STAGES
摘要
Abstract
In rice the period of grain filling is characterized by transition of all vegetative organs from sink to source. A pot experiment was conducted to monitor variation of N and protein concentrations, N translocation and enzyme activities representative of nitrogen metabolism after the heading stage in different leaves and stem sections of two rice cultivars different in N use efficiency (NUE), high and low, treated with two levels of N fertilization.Compared with CK or Treatment No, N application increased N concentration, soluble protein concentration and N transloeation in leaves and stems. Nitrogen concentration in functional leaves decreased by 60％ -67％ from heading to maturity, regardless of N rates, which indicates that N in grains comes mainly from leaves. However, N contribution rate from stems to grain depends on environmental N supply. N and soluble protein concentration and N translocation in leaves and stems was lower in Nanguang than in Elio at the maturity stage. Compared with CK, N application increased the activities of four N enzymes. And the activities decreased with the plant growth. The NR and GS activities were higher in Nanguang than in Elio, while NR activity depends more on N rate and growth stage. The GOGAT and GDH activities were lower in Nanguang than in Elio. NR and GS activities were significantly correlated to N and soluble protein concentrations at the heading stage and 2 weeks after the heading. All these indicate that higher NR and GS activities especially the latter, may predict the N status of rice, high N transloeation and high NUE in rice.关键词
水稻/氮效率/氮浓度/氮代谢酶活性Key words
Rice/ N use efficiency/ N concentration/ Activities of N metabolizing enzymes分类
农业科技引用本文复制引用
叶利庭,吕华军,宋文静,图尔迪,沈其荣,张亚丽..不同氮效率水稻生育后期氮代谢酶活性的变化特征[J].土壤学报,2011,48(1):132-140,9.基金项目
国家自然科学基金项目(30771290和31071846)、国家大学生创新性实验计划(091030725)、中国博士后科学基金(20100471348)资助 （30771290和31071846）
