安徽农业科学2011，Vol.39Issue(1)：225-227,3.
马铃薯炭疽病菌分离与鉴定
Isolation and Identification of Colletotrichum coccodes
张建成 1张慧丽 1顾建锋1
作者信息
- 1. 宁波出入境检验检疫局,浙江宁波,315012
- 折叠
摘要
Abstract
[ Objective] The paper aimed to separate and identify pathogenic fungi from sick potato tubers what carried by entry ships, make clear the significance of quarantine and study the rapid identification method of germs. [ Method] Based on the morphological observation and ITS sequence analysis, germs were identified, and the pre-existing nested PCR method has been verified. [ Result] The morphological observation and ITS sequence analysis indicated that the germs were Colletotrichum coccodes. Based on nested PCR method, the spocies-specific primers ( CclNFl/Cc2NRl ) amplified C. coccodes, so 349 bp specific fragments were obtained. [ Conclusion] The nested PCR method could be used for the rapid identification of C. coccodes. It is the potential dangerous fungus of entry plant quarantine. It is the first time to intercept C. coccodes in domestic port.关键词
马铃薯炭疽病菌/鉴定/巢式PCRKey words
Colletotrichum coccodes/ Identification/ Nested PCR分类
农业科技引用本文复制引用
张建成,张慧丽,顾建锋..马铃薯炭疽病菌分离与鉴定[J].安徽农业科学,2011,39(1):225-227,3.
