安徽农业科学2011，Vol.39Issue(1)：236-237,240,3.
紫外分光光度法测定橙汁中维生素C的含量
Deternmination of Vitamin C in Orange Juice by UV Spectrophotometric Method
摘要
Abstract
[Objective] Tth aim was to provide refereneces for the scientific determination of Vitamin C (Vc) in sold beverage. [Method]With Cu2+ as catalyst to oxidize Vc, the method to determine reduced Vc in orange juice by UV spectrophotometry was constructed, and then the determination conditions were optimized. [ Result] With the mixed solution of phosphoric acid and acetic acid (3∶7, V∶ V) as the stabiliz er, Cu2+ as catalyst and in the 70 ℃ water-circulator bath, Vc content in the sold orange juice was determined, the standard curve equation was y = 0.071 4x + 0.135 0, the linear correlation coefficient was 0.999 5, the precision RSD( n = 5 ) was 0.35％ and recovery rate was 98.5％. [ Conclusion] The method to determine Vc in orange juice by UV spectrophotometry was constructed, which was simple and fiast and whose results were very satisfying.关键词
维生素C/紫外分光光度法/橙汁Key words
Vitamin C/ UV spectrophotometry/ Orange juice分类
农业科技引用本文复制引用
陈玉锋,庄志萍..紫外分光光度法测定橙汁中维生素C的含量[J].安徽农业科学,2011,39(1):236-237,240,3.基金项目
牡丹江师范学院指导项目(KZ2008008) （KZ2008008）
牡丹江师范学院重点项目(Z2008003). （Z2008003）
